米象GST基因的表达及其对磷化氢的应答

摘要分析米象代谢解毒相关基因谷胱甘肽硫转移酶（GST）的表达，获得米象基因遗传信息，进而从本质上全面揭示米象的磷化氢抗性。米象敏感品系经磷化氢不同浓度熏蒸和不同时间熏蒸处理，2个基因经LC30浓度磷化氢熏蒸的相对表达量显著高于其他2个浓度；4个GSTs基因经LC50浓度磷化氢熏蒸的相对表达量显著高于对照组和LC30、LC10处理组；3个基因相对表达量均显著低于对照组。7个GSTs基因随着熏蒸时间的增加，相对表达量逐渐升高。米象GSTs基因在不同发育阶段的相对表达模式中，SoGSTd1基因在四龄幼虫阶段的相对表达量最高；SoGSTe1基因在成虫阶段的相对表达量最高；SoGSTe2和SoGSTe5基因在四龄幼虫阶段的相对表达量最大，SoGSTe6基的相对表达量在三龄幼虫阶段最大。米象13个GSTs基因在不同组织部位的相对表达模式中。米象13个GSTs基因中的11个在中肠、脂肪体或马氏管中的相对表达量均显著高于头部和表皮中的表达量。

关键词 米象；谷胱甘肽硫转移酶；磷化氢；基因表达

中图分类号S433.5文献标识码A

文章编号0517-6611（2019）08-0114-05

doi：10.3969/j.issn.0517-6611.2019.08.029

AbstractThrough transcriptome technology，several resistant genes related to S.oryzae such as glutathione Stransferase，cytochrome were analyzed and the transcriptome genetic information was obtained，revealing the resistance of insects in nature at transcriptome level is gaining a huge interest among the researchers.The S.oryzae sensitive strains of different concentrations of phosphine fumigation and fumigation treatment at different time were investiagted.The concentration of two genes was significantly higher than the other two concentrations by the concentration of LC30，and the relative expression of the four GSTs genes was significantly higher than that of the control group and the LC30，LC10 treatment group，and the relative expression of the three genes was significantly lower than that of the control group.The relative expression level of 7 GSTs genes increased gradually with the increase of fumigation time.The relative expression of the SoGSTd1 in the fourinstar stage was the highest in the relative expression pattern of the GSTs gene in the different developmental stages，and the relative expression of the SoGSTe1 gene in the adult stage was the highest.The relative expression of the SoGSTe2 and SoGSTe5 genes in the four instar stage was the largest，and the relative expression of the SoGSTe6 gene in the three instar larvae was maximum.13 GSTs genes of S.oryzae were differently expression in different tissues.The relative expression level of 13 GSTs genes in midgut，fat body or malpighian tubules in S.oryzae was significantly higher than that in the head and cuticle.The relative expression level of 11 genes in the midgut，fat body or malpighian tubules was higher than that in the head and cuticle.

Key wordsSitophilus oryzae；Glutathione Stransferases；Phosphine；Gene expression

米象（Sitophilus oryzae）屬于鞘翅目，象虫科，是一种完全变态昆虫。它有卵、幼虫、蛹、成虫4个发育形态，是贮藏谷物的主要害虫之一，主要寄生在贮存2～3年的陈粮中，例如玉米、水稻、小麦、高粱等谷物中，成虫在外部啃食谷物颗粒，幼虫则寄生在谷物内部蛀食[1]。由于其地理分布遍布全世界，生长繁殖速度快，因此对贮藏谷物危害严重[2]。在我国，米象主要生长在南方地区。

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